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| April 23, 2010 - June 19 - 24, 2010: Orochem Technologies will be attending HPLC 2010 to be held in Boston. | | | | April 23, 2010 - May 24 - 26, 2010: Orochem Technologies will be attending ASM 2010 to be held in San Diego. | | | | April 23, 2010 - May 9 - 11, 2010: Orochem Technologies will be attending PREP 2010 to be held in Philadelphia. | | |
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1. Additional Materials Required
- Variable-speed bench-top microcentrifuge
- 1.5-2.0 ml microcentrifuge collection tubes
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2. Sample Loading Guidelines
- Sample purity and yield obtained after desalting is largely dependent on the sample loading volume. For optimal results, load sample volumes from 30 to 120 µl. If sample is of high ionic strength, such as 0.5-1M CaCl2, reduce maximum sample volume to 75 µl.
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3. Protein Desalting Spin Column Preparation
- Invert column to suspend slurry.
- Loosen the cap.
- Place column in 1.5-2.0 ml microcentrifuge collection tube.
- Centrifuge at 1,500 x g for 1 minute to remove excess liquid.
- Blot bottom of column on a paper towel to remove any excess-trapped liquid.
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4. Sample Loading
- Place column in a fresh collection tube, remove cap and apply 30-120 µl of sample to the center of the compacted resin bed.
- (Optional) To improve recovery percentage of low molecular weight proteins or for small sample volumes, add 20-40 µl of 10 mM Tris Buffer, pH 7.5, to top of resin. Do not exceed a total sample plus stacker volume of 120 µl.
- Centrifuge at 1,500 x g for 2 minutes. The desalted sample is collected in collection tube.
- Discard desalting column after use.
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