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Register online and avail up to 20% off of on all HPLC columns. Contact us today at 630.916.0225 for details
 
OroSym™ HPLC Column
 
OroSym™ C18 column series are bonded to the maximum density to have the highest hydrophobicity and thus the lowest polarity. High C18 moieties result in the lowest interaction with analytes, with silanol, and demonstrate high stability at high pH and the broadest pH range. OroSym™ columns also demonstrate higher separation power for isomers, with a near perfect symmetry for basic, acidic and neutral compounds
Features:
  • Surface Area: 45m²/g
  • Pore Size: 130Å
Selectivity of Basic, Acidic and Neutral Compounds



  • p-methyl benzoic acid
  • Pyridine
  • Phenol
  • Acetophenone
  • Amino-dimethyl benzene
  • Toluene

150 x 4.6 mm column at 30°C
Mobile Phase: MeOH: AcOH/ NaAc(pH 5.0)= 60 : 40
Flow Rate: 1 mL/min
20μL injection detected at 254 nm


Figure 2: Analysis on Orosil ODS 250x4.6mm, 5μm

Selectivity of Basic Compounds


150 x 4.6 mm column at 30°C
Mobile Phase: MeOH: NaH2PO4/ Na2H PO4(pH 7.0)=70 : 30
Flow Rate: 1 mL/min
10μL injection detected at 254 nm
Test of Relative Polarity
Sample: 1. Uracil 2. Pyridine 3. Phenol
Promosil C18
KPyridine/KPhenol = 0.33
MP C18
KPyridine/KPhenol = 0.45
Luna C18
KPyridine/KPhenol = 0.5
Orosym C18
KPyridine/KPhenol = 0.53
OASB C18
KPyridine/KPhenol = 0.82

150 x 4.6 mm column at 30°C
Mobile Phase: MeOH: water = 60:40
Flow Rate: 1ml/min
10µl injection detected at 254nm

Test of Metal Effects
Sample: 1. 2, 7- dihdroxynaphthalene
2. 2, 3- dihydroxynaphthalene


150 x 4.6 mm column at 30°C
MeOH: water = 65 : 35
Flow Rate: 1 mL/min
20μL injection detected at 230 nm

Test of Hydrophobicity
Sample: 1. Urasil 2. Toluene


150 x 4.6 mm column at 25°C
MeOH : 25mM KH2PO4 /K2HPO4 (pH 6.0) = 80 : 20
Flow Rate: 1 mL/min
5μL injection detected at 254 nm

Test of Lifetime under pH 1.5
Sample: 1. Uracil 2. Phenol 3. Nitrobenzene 4. Naphthalene


150 x 4.6 mm column at 50°C
MeOH : 0.25% TFA (pH 1.5) = 70 : 30
Flow Rate: 1 mL/min
5μL injection detected at 254 nm
 
Ordering Information
 
  3μm

  Phase Size Product Code Price($)
  3 µm C8 100 x 4.6 mm OY3DI-922 Please Register to view Price         
  3 µm C18 100 x 4.6 mm OY3DI-920 Please Register to view Price         
  3 µm C8 50 x 4.6 mm OY3BI-922 Please Register to view Price         
  3 µm C18 50 x 4.6 mm OY3BI-920 Please Register to view Price         
  3 µm C8 150 x 2.0 mm OY3FI-922 Please Register to view Price         
  3 µm C18 150 x 2.0 mm OY3FI-920 Please Register to view Price         
  3 µm C8 50 x 3.0 mm OY3BG-922 Please Register to view Price         
  3 µm C18 50 x 3.0 mm OY3BG-920 Please Register to view Price         
 
  5μm

  Phase Size Product Code Price($)
  5 µm C8 50 x 3.0 mm OY5BG-921 Please Register to view Price         
  5 µm C18 50 x 3.0 mm OY5BG-919 Please Register to view Price         
  5 µm C8 50 x 4.6 mm OY5BI-921 Please Register to view Price         
  5 µm C18 50 x 4.6 mm OY5BI-919 Please Register to view Price         
  5 µm C8 50 x 2.0 mm OY5BH-921 Please Register to view Price         
  5 µm C18 50 x 2.0 mm OY5BH-919 Please Register to view Price         
  5 µm C8 150 x 4.6 mm OY5FI-921 Please Register to view Price         
  5 µm C18 150 x 4.6 mm OY5FI-919 Please Register to view Price         
  5 µm C8 125 x 3.0 mm OY5EH-921 Please Register to view Price         
  5 µm C18 125 x 3.0 mm OY5EH-919 Please Register to view Price         
  5 µm C8 250 x 4.6 mm OY5HI-921 Please Register to view Price         
  5 µm C18 250 x 4.6 mm OY5HI-919 Please Register to view Price         
 
 
 
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