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February 08, 2010 - Feb 16-19,2010: Dr. Asha Oroskar & Dr. Anil Oroskar, will be attending "InformEx" meeting held at San Francisco,CA. Connect to Link
 
February 08, 2010 - Feb 8th & 9th, 2010 : Orochem Technologies Inc. attending the "Next Generagtion Bio-Based Chemicals Summit " held at San Diego,CA. The CTO, Dr. Anil Oroskar, will be representing Orochem & is available to meet with interested parties. Connect to Link
 
July 03, 2009 - Orochem Technologies Inc. is exhibiting at AACC Clinical Lab Expo 2009 at McCormic Place Covention Center, Chicago, IL from July 21 - 23, 2009. Welcome to Orochem Booth #4339.
 
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Register online and avail up to 20% off of on all HPLC columns. Contact us today at 630.916.0225 for details
 
 
 
Guard Cartridge
 
Guard cartridges are protection devices for analytical and preparative HPLC columns. They function as a filter and adsorber of materials that can adversely affect the chemistry of the separation or the lifetime of the column. Their use is highly recommended when injecting anything other than pure filtered standards that elute in a reasonable number (<20) of column volumes.

The best way to correctly choose the appropriate guard cartridge is to first consider its objective in the system. There are a multitude of designs and sizes available, and proper choice will provide maximum benefit with minimal effects to the chromatography.

Kept in mind that guard cartridges are small columns, and as such they will have backpressure and will contribute to the separation resolution. Because of the effect, even though it is minor, that guard cartridges have on the separation, it is always advised that the stationary phase inside the guard cartridge be the same as that in the analytical column to avoid unexpected results. Typically it is best to use guard cartridges with internal diameters comparable to that of the analytical column. This insures that the linear capacity of the cartridge is adequate for the expected column injection sizes and that the contribution to backpressure is minimal.

Frequently chromatographers will choose guard cartridges packed with media of a larger particle size than that in the HPLC column. This also helps to reduce the backpressure contribution from the guard cartridge. Since the length of the guard cartridge packed bed is typically less than 1/10th of the HPLC column, its contribution to the overall efficiency and symmetry is negligible unless the difference in particle sizes between that used in the column and that used in the guard cartridge is greater than an order of magnitude.

Since the guard cartridge is like a small HPLC column, proper care must be taken in plumbing it into the system to avoid an increase in extra dead volume. Use minimal tubing lengths from injector to guard cartridge and from guard cartridge to column.

The most common question regarding guard cartridges is when they should be changed. If the backpressure increases more than 10% beyond that observed with a fresh guard cartridge, change the cartridge. If a noticeable deterioration of the chromatography occurs after several injections, change the guard cartridge.

In summary, guard cartridges are a valuable tool, and with proper use they can help to extend HPLC column life significantly without any adverse effects.
     
OROCHEM GUARD COLUMN
 
Orochem guard columns protect your analytical HPLC column. They remove particulate contaminants and highly adsorptive compounds from samples, prolonging analytical column life.
  • Guard columns are modular and cartridge replacement is easy.
  • Guard column cartridge holders are available in stainless steel and PEEK materials.
  • Both designs are optimized to reduce dead volume and prevent extra column band broadening.
  • Holders for the cartridges are reusable indefinitely.
 
ADVANTAGES OF USING OROCHEM GUARD COLUMN
  • Higher bed volumes, enhance performance
  • Virtually no change in chromatography.
  • Available in analytical, semi-prep and preparative sizes.
  • Extensive range of chemistries.
 
EZGUARD DELUXE™
 
Dimension
  • 3.0 x 15mm
  • 2.1 x 15mm
  • 1.0 x 15mm
 Holder
 
EZGUARD™
 
1mm 2mm
 
EZGUARD EXPRESS&trade

MATRIX

 
  Phase Size Product
  3 μm NH2 10 x 2.0 mm R3-138-2
  3 μm C8 10 x 2.0 mm R3-135-2
  3 μm AQ 10 x 2.0 mm R3-171-2
  3 μm C18 10 x 2.0 mm R3-136-2
  3 μm NH2 10 x 4.0 mm R3-138-4
  3 μm C8 10 x 4.0 mm R3-135-4
  3 μm AQ 10 x 4.0 mm R3-171-4
  3 μm C18 10 x 4.0 mm R3-136-4
  3 μm NH2 10 x 10.0 mm R3-138-10
  3 μm C8 10 x 10.0 mm R3-135-10
  3 μm AQ 10 x 10.0 mm R3-171-10
  3 μm C18 10 x 10.0 mm R3-136-10
  3 μm NH2 20 x 20.0 mm R3-138-20
  3 μm C8 20 x 20.0 mm R3-135-20
  3 μm AQ 20 x 20.0 mm R3-171-20
  3 μm C18 20 x 20.0 mm R3-136-20
  5 μm NH2 10 x 2.0 mm R5-137-2
  5 μm C8 10 x 2.0 mm R5-130-2
  5 μm C18 AQ 10 x 2.0 mm R5-131-2
  5 μm C18 10 x 2.0 mm R5-170-2
  5 μm CN 10 x 2.0 mm R5-428-2
  5 μm NH2 10 x 4.0 mm R5-137-4
  5 μm C8 10 x 4.0 mm R5-130-4
  5 μm C18 AQ 10 x 4.0 mm R5-131-4
  5 μm C18 10 x 4.0 mm R5-170-4
  5 μm CN 10 x 4.0 mm R5-428-4
  5 μm NH2 10 x 10.0 mm R5-137-10
  5 μm C8 10 x 10.0 mm R5-130-10
  5 μm C18 AQ 10 x 10.0 mm R5-13110
  5 μm C18 10 x 10.0 mm R5-170-10
  5 μm CN 10 x 10.0 mm R5-428-10
  5 μm NH2 20 x 20.0 mm R5-137-20
  5 μm C8 20 x 20.0 mm R5-130-20
  5 μm C18 AQ 20 x 20.0 mm R5-131-20
  5 μm C18 20 x 20.0 mm R5-170-20
  5 μm CN 20 x 20.0 mm R5-428-20
  10 μm CN 10 x 2.0 mm R10-139-2
  10 μm C8 10 x 2.0 mm R10-144-2
  10 μm C18 10 x 2.0 mm R10-146-2
  10 μm C18 AQ 10 x 2.0 mm R10-172-2
  10 μm CN 10 x 4.0 mm R10-139-4
  10 μm C8 10 x 4.0 mm R10-144-4
  10 μm C18 10 x 4.0 mm R10-146-4
  10 μm C18 AQ 10 x 4.0 mm R10-172-4
  10 μm CN 10 x 10.0 mm R10-139-10
  10 μm C8 10 x 10.0 mm R10-144-10
  10 μm C18 10 x 10.0 mm R10-146-10
  10 μm C18 AQ 10 x 10.0 mm R10-172-10
  10 μm CN 20 x 20.0 mm R10-139-20
  10 μm C8 20 x 20.0 mm R10-144-20
  10 μm C18 20 x 20.0 mm R10-146-20
  10 μm C18 AQ 20 x 20.0 mm R10-172-20

MONITOR

 
  Phase Size Product
  3 μm NH2 10 x 2.0 mm M3 -016-2
  3 μm C8 10 x 2.0 mm M3 -002-2
  3 μm C18 10 x 2.0 mm M3 -001-2
  3 μm CN 10 x 2.0 mm M3 -015-2
  3 μm Silica 10 x 2.0 mm M3 -003-2
  3 μm NH2 10 x 4.0 mm M3-016-4
  3 μm C8 10 x 4.0 mm M3-002-4
  3 μm C18 10 x 4.0 mm M3-001-4
  3 μm CN 10 x 4.0 mm M3-015-4
  3 μm Silica 10 x 4.0 mm M3-003-4
  3 μm NH2 10 x 10.0 mm M3-016-10
  3 μm C8 10 x 10.0 mm M3-002-10
  3 μm C18 10 x 10.0 mm M3-001-10
  3 μm CN 10 x 10.0 mm M3-015-10
  3 μm Silica 10 x 10.0 mm M3-003-10
  3 μm NH2 20 x 20.0 mm M3-016-20
  3 μm C8 20 x 20.0 mm M3-002-20
  3 μm C18 20 x 20.0 mm M3-001-20
  3 μm CN 20 x 20.0 mm M3-015-20
  3 μm Silica 20 x 20.0 mm M3-003-20
  5 μm NH2 10 x 2.0 mm M5-017-2
  5 μm C8 10 x 2.0 mm M5- 006-2
  5 μm C18 10 x 2.0 mm M5 -005-2
  5 μm CN 10 x 2.0 mm M5 -013-2
  5 μm Silica 10 x 2.0 mm M5 -004-2
  5 μm NH2 10 x 4.0 mm M5-017-4
  5 μm C8 10 x 4.0 mm M5-006-4
  5 μm C18 10 x 4.0 mm M5-005-4
  5 μm CN 10 x 4.0 mm M5-013-4
  5 μm Silica 10 x 4.0 mm M5-004-4
  5 μm NH2 10 x 10.0 mm M5-017-10
  5 μm C8 10 x 10.0 mm M5-006-10
  5 μm C18 10 x 10.0 mm M5-005-10
  5 μm CN 10 x 10.0 mm M5-013-10
  5 μm Silica 10 x 10.0 mm M5-004-10
  5 μm NH2 20 x 20.0 mm M5-017-20
  5 μm C8 20 x 20.0 mm M5-006-20
  5 μm C18 20 x 20.0 mm M5-005-20
  5 μm CN 20 x 20.0 mm M5-013-20
  5 μm Silica 20 x 20.0 mm M5-004-20
  10 μm NH2 10 x 2.0 mm M10-018-2
  10 μm C8 10 x 2.0 mm M10-008-2
  10 μm C18 10 x 2.0 mm M10-009-2
  10 μm CN 10 x 2.0 mm M10-020-2
  10 μm Silica 10 x 2.0 mm M10-007-2
  10 μm NH2 10 x 4.0 mm M10-018-4
  10 μm C8 10 x 4.0 mm M10-008-4
  10 μm C18 10 x 4.0 mm M10-009-4
  10 μm CN 10 x 4.0 mm M10-020-4
  10 μm Silica 10 x 4.0 mm M10-007-4
  10 μm NH2 10 x 10.0 mm M10-018-10
  10 μm C8 10 x 10.0 mm M10-008-10
  10 μm C18 10 x 10.0 mm M10-009-10
  10 μm CN 10 x 10.0 mm M10-020-10
  10 μm Silica 10 x 10.0 mm M10-007-10
  10 μm NH2 20 x 20.0 mm M10-018-20
  10 μm C8 20 x 20.0 mm M10-008-20
  10 μm C18 20 x 20.0 mm M10-009-20
  10 μm CN 20 x 20.0 mm M10-020-20
  10 μm Silica 20 x 20.0 mm M10-020-20

PRESTIGE

 
  Phase Size Product
  3 μm C8 10 x 2.0 mm P3-072-2
  3 μm C18 10 x 2.0 mm P3-073-2
  3 μm C8 10 x 4.0 mm P3-072-4
  3 μm C18 10 x 4.0 mm P3-073-4
  3 μm C8 10 x 10.0 mm P3-072-10
  3 μm C18 10 x 10.0 mm P3-073-10
  3 μm C8 20 x 20.0 mm P3-072-20
  3 μm C18 20 x 20.0 mm P3-073-20
  5 μm C8 10 x 2.0 mm P5-071-2
  5 μm C18 10 x 2.0 mm P5-070-2
  5 μm C8 10 x 4.0 mm P5-071-4
  5 μm C18 10 x 4.0 mm P5-070-4
  5 μm C8 10 x 10.0 mm P5-071-10
  5 μm C18 10 x 10.0 mm P5-070-10
  5 μm C8 20 x 20.0 mm P5-071-20
  5 μm C18 20 x 20.0 mm P5-070-20
  10 μm C8 10 x 2.0 mm P10-074-2
  10 μm C18 10 x 2.0 mm P10-075-2
  10 μm C8 10 x 4.0 mm P10-074-4
  10 μm C18 10 x 4.0 mm P10-075-4
  10 μm C8 10 x 10.0 mm P10-074-10
  10 μm C18 10 x 10.0 mm P10-075-10
  10 μm C8 20 x 20.0 mm P10-074-20
  10 μm C18 20 x 20.0 mm P10-075-20

RELIASIL

 
  Phase Size Product
  3 µm NH2 10 x 2.0 mm R3-103-2
  3 µm C8 10 x 2.0 mm R3-102-2
  3 µm C18 10 x 2.0 mm R3-101-2
  3 µm CN 10 x 2.0 mm R3-105-2
  3 µm Silica 10 x 2.0 mm R3-100-2
  3 µm SCX 10 x 2.0 mm R3-107-2
  3 µm NH2 10 x 4.0 mm R3-103-4
  3 µm C8 10 x 4.0 mm R3-102-4
  3 µm C18 10 x 4.0 mm R3-101-4
  3 µm CN 10 x 4.0 mm R3-105-4
  3 µm Silica 10 x 4.0 mm R3-100-4
  3 µm SCX 10 x 4.0 mm R3-107-4
  3 µm NH2 10 x 10.0 mm R3-103-10
  3 µm C8 10 x 10.0 mm R3-102-10
  3 µm C18 10 x 10.0 mm R3-101-10
  3 µm CN 10 x 10.0 mm R3-105-10
  3 µm Silica 10 x 10.0 mm R3-100-10
  3 µm SCX 10 x 10.0 mm R3-107-10
  3 µm NH2 20 x 20.0 mm R3-103-20
  3 µm C8 20 x 20.0 mm R3-102-20
  3 µm C18 20 x 20.0 mm R3-101-20
  3 µm CN 20 x 20.0 mm R3-105-20
  3 µm Silica 20 x 20.0 mm R3-100-20
  3 µm SCX 20 x 20.0 mm R3-107-20
  5 µm NH2 10 x 2.0 mm R5-132-2
  5 µm C8 10 x 2.0 mm R5-122-2
  5 µm C18 10 x 2.0 mm R5-121-2
  5 µm CN 10 x 2.0 mm R5-125-2
  5 µm Silica 10 x 2.0 mm R5-120-2
  5 µm SCX 10 x 2.0 mm R5-133-2
  5 µm NH2 10 x 4.0 mm R5-132-4
  5 µm C8 10 x 4.0 mm R5-122-4
  5 µm C18 10 x 4.0 mm R5-121-4
  5 µm CN 10 x 4.0 mm R5-125-4
  5 µm Silica 10 x 4.0 mm R5-120-4
  5 µm SCX 10 x 4.0 mm R5-133-4
  5 µm NH2 10 x 10.0 mm R5-132-10
  5 µm C8 10 x 10.0 mm R5-122-10
  5 µm C18 10 x 10.0 mm R5-121-10
  5 µm CN 10 x 10.0 mm R5-125-10
  5 µm Silica 10 x 10.0 mm R5-120-10
  5 µm SCX 10 x 10.0 mm R5-133-10
  5 µm NH2 20 x 20.0 mm R5-132-20
  5 µm C8 20 x 20.0 mm R5-122-20
  5 µm C18 20 x 20.0 mm R5-121-20
  5 µm CN 20 x 20.0 mm R5-125-20
  5 µm Silica 20 x 20.0 mm R5-120-20
  5 µm SCX 20 x 20.0 mm R5-133-20

RELIACHROM

 
  Phase Size Product
  3 μm NH2 10 x 2.0 mm RC3-937-2
  3 μm C8 10 x 2.0 mm RC3-949-2
  3 μm C18 10 x 2.0 mm RC3-952-2
  3 μm CN 10 x 2.0 mm RC3-946-2
  3 μm Silica 10 x 2.0 mm RC3-930-2
  3 μm SCX 10 x 2.0 mm RC3-947-2
  3 μm NH2 10 x 4.0 mm RC3-937-4
  3 μm C8 10 x 4.0 mm R3C-949-4
  3 μm C18 10 x 4.0 mm R3C-952-4
  3 μm CN 10 x 4.0 mm R3C-946-4
  3 μm Silica 10 x 4.0 mm R3C-930-4
  3 μm SCX 10 x 4.0 mm R3C-940-4
  3 μm NH2 10 x 10.0 mm RC3-937-10
  3 μm C8 10 x 10.0 mm RC3-949-10
  3 μm C18 10 x 10.0 mm RC3-952-10
  3 μm CN 10 x 10.0 mm RC3-946-10
  3 μm Silica 10 x 10.0 mm RC3-930-10
  3 μm SCX 10 x 10.0 mm RC3-940-10
  3 μm NH2 20 x 20 mm RC3-937-20
  3 μm C8 20 x 20 mm RC3-949-20
  3 μm C18 20 x 20 mm RC3-952-20
  3 μm CN 20 x 20 mm RC3-946-20
  3 μm Silica 20 x 20 mm RC3-930-20
  3 μm SCX 20 x 20 mm RC3-940-20
  5 μm NH2 10 x 2.0 mm RC5-938-2
  5 μm C8 10 x 2.0 mm RC5-950-2
  5 μm C18 10 x 2.0 mm RC5-953-2
  5 μm CN 10 x 2.0 mm RC5-947-2
  5 μm SCX 10 x 2.0 mm RC5-941-2
  5 μm Silica 10 x 2.0 mm RC5-931-2
  5 μm NH2 10 x 4.0 mm RC5-938-4
  5 μm C8 10 x 4.0 mm RC5-950-4
  5 μm C18 10 x 4.0 mm RC5-953-4
  5 μm CN 10 x 4.0 mm RC5-947-4
  5 μm SCX 10 x 4.0 mm RC5-941-4
  5 μm Silica 10 x 4.0 mm RC5-931-4
  5 μm NH2 10 x 10.0 mm RC5-938-10
  5 μm C8 10 x 10.0 mm RC5-950-10
  5 μm C18 10 x 10.0 mm RC5-953-10
  5 μm CN 10 x 10.0 mm RC5-947-10
  5 μm SCX 10 x 10.0 mm RC5-941-10
  5 μm Silica 10 x 10.0 mm RC5-931-10
  5 μm NH2 20 x 20.0 mm RC5-938-20
  5 μm C8 20 x 20.0 mm RC5-950-20
  5 μm C18 20 x 20.0 mm RC5-953-20
  5 μm CN 20 x 20.0 mm RC5-947-20
  5 μm SCX 20 x 20.0 mm RC5-941-20
  5 μm Silica 20 x 20.0 mm RC5-931-20
  10 μm NH2 10 x 2.0 mm RC10-939-2
  10 μm C8 10 x 2.0 mm RC10-951-2
  10 μm C18 10 x 2.0 mm RC10-954-2
  10 μm CN 10 x 2.0 mm RC10-948-2
  10 μm Silica 10 x 2.0 mm RC10-932-2
  10 μm NH2 10 x 4.0 mm RC10-939-4
  10 μm C8 10 x 4.0 mm RC10-951-4
  10 μm C18 10 x 4.0 mm RC10-954-4
  10 μm CN 10 x 4.0 mm RC10-948-4
  10 μm Silica 10 x 4.0 mm RC10-932-4
  10 μm NH2 10 x 10.0 mm RC10-939-10
  10 μm C8 10 x 10.0 mm RC10-951-10
  10 μm C18 10 x 10.0 mm RC10-954-10
  10 μm CN 10 x 10.0 mm RC10-948-10
  10 μm Silica 10 x 10.0 mm RC10-932-10
  10 μm NH2 20 x 20.0 mm RC10-939-20
  10 μm C8 20 x 20.0 mm RC10-951-20
  10 μm C18 20 x 20.0 mm RC10-954-20
  10 μm CN 20 x 20.0 mm RC10-948-20
  10 μm Silica 20 x 20.0 mm RC10-932-20
 
 
 
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