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| April 23, 2010 - June 19 - 24, 2010: Orochem Technologies will be attending HPLC 2010 to be held in Boston. | | | | April 23, 2010 - May 24 - 26, 2010: Orochem Technologies will be attending ASM 2010 to be held in San Diego. | | | | April 23, 2010 - May 9 - 11, 2010: Orochem Technologies will be attending PREP 2010 to be held in Philadelphia. | | |
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Register online and avail up to 20% off of on all HPLC columns. Contact us today at 630.916.0225 for details
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| Orochem has the following purification options available to remove the impurities generated during the synthesis of modified oligonucleotides. Which purification method is employed depends on how the oligonucleotide is modified and how it will be used |
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| Desalting procedures remove inorganic salts, traces of organic compounds, low molecular weight impurities, and short failure sequences. Desalting is accomplished by taking advantage of size (molecular weight) or solubility differences. |
| Gel filtration removes low molecular weight molecules, inorganic salts and very short failure sequences. Purification occurs by passing a sample through a Sephadex® column, which separates molecules based on size. |
These cartridges work on the principle of adsorption of the DMT group of the trityl-on oligonucleotide while non-DMT bearing failure sequences, by-products, and other impurities wash through. The DMT group is removed with mild acid, allowing the purified, detritylated oligonucleotide to be eluted.
These cartridge not only eliminates salts and trace organic impurities from the crude oligonucleotide, but also provides pure oligonucleotides for sequencing primers, PCR primers, hybridization probes, and gene constructions. |
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- Loading of the oligonucleotide in a 0.1 M triethyl ammonium acetate (TEAA)
- Elution of trityl-off failures in 15% acetonitrile/ 85% 0.1MTEAA
- Detritylation using 0.5% trifluoroacetic acid (TFA)
- Elution of trityl-off oligo product using acetonitrile gradient
- Wash with 100% acetonitrile
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