 |
|
 |
 |
|
|
|
|
|
 Latest News |
| |
| February 08, 2010 - Feb 16-19,2010: Dr. Asha Oroskar & Dr. Anil Oroskar, will be attending "InformEx" meeting held at San Francisco,CA. Connect to Link | | | | February 08, 2010 - Feb 8th & 9th, 2010 : Orochem Technologies Inc. attending the "Next Generagtion Bio-Based Chemicals Summit " held at San Diego,CA. The CTO, Dr. Anil Oroskar, will be representing Orochem & is available to meet with interested parties. Connect to Link | | | | July 03, 2009 - Orochem Technologies Inc. is exhibiting at AACC Clinical Lab Expo 2009 at McCormic Place Covention Center, Chicago, IL from July 21 - 23, 2009. Welcome to Orochem Booth #4339. | | |
|
| |
More News... |
|
Technical Corner |
| |
Catalog
|
| |
Application Notes
|
| |
Handbooks & Protocols
|
| |
Newsletters
|
|
Special Offers |
| |
Register online and avail up to 20% off of on all HPLC columns. Contact us today at 630.916.0225 for details
|
|
| |
| |
| |
| |
| Orochem has the following purification options available to remove the impurities generated during the synthesis of modified oligonucleotides. Which purification method is employed depends on how the oligonucleotide is modified and how it will be used |
| |
| Desalting procedures remove inorganic salts, traces of organic compounds, low molecular weight impurities, and short failure sequences. Desalting is accomplished by taking advantage of size (molecular weight) or solubility differences. |
| Gel filtration removes low molecular weight molecules, inorganic salts and very short failure sequences. Purification occurs by passing a sample through a Sephadex® column, which separates molecules based on size. |
These cartridges work on the principle of adsorption of the DMT group of the trityl-on oligonucleotide while non-DMT bearing failure sequences, by-products, and other impurities wash through. The DMT group is removed with mild acid, allowing the purified, detritylated oligonucleotide to be eluted.
These cartridge not only eliminates salts and trace organic impurities from the crude oligonucleotide, but also provides pure oligonucleotides for sequencing primers, PCR primers, hybridization probes, and gene constructions. |
|
 |
|
- Loading of the oligonucleotide in a 0.1 M triethyl ammonium acetate (TEAA)
- Elution of trityl-off failures in 15% acetonitrile/ 85% 0.1MTEAA
- Detritylation using 0.5% trifluoroacetic acid (TFA)
- Elution of trityl-off oligo product using acetonitrile gradient
- Wash with 100% acetonitrile
|
| |
|
| |
| |
| |
|
|
| |
|
|
|
|