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Orochem Aquamatrix plates are 96 well plates in 1mL or 2mL formats packed with flux calcined diatomaceous earth for high capacity retention of analytes.

Features and Applications:

Packed with chemically inert material, stable in the pH range 1-13
Plates compatible with robotic liquid handlers for automated extraction
Allows high throughput alternative to traditional liquid-liquid extraction techniques
Eliminates steps like mixing, centrifugation and organic phase separation
Provides support, greater surface area and accelerated solvent extraction
No pre-treatment of the bed is necessary

Ordering Information

Aquamatrix 96-well Plates

Catalog #	Descriptions	Qty/Pkg.	List Price

OC21DA260 -HPGB

Unbuffered Flux Calcined Diatomaceous Earth 260 mg 2mL plate

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OMNDA150 - HPGB

Unbuffered Flux Calcined Diatomaceous Earth 150 mg 1mL plate

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OC21DA400 -HPGB

Unbuffered Flux Calcined Diatomaceous Earth 400 mg 2mL plate

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Also Available with Diatomaceous Earth at buffered pH of 4.5 and 9.0

Recommended Protocol

A mixture of sample (e.g., plasma), internal standard, and buffer solution to adjust pH is prepared.
This aqueous mixture is then added to the dry particle bed (no conditioning or pretreatment of the bed is necessary).
The mixture is allowed to partition for about 3-5 minutes on the particle surface under gravity flow.
The analyte in aqueous solution is now spread out among the particles and occupies a high surface area. A hydrophobic filter at the bottom of each well prevents the aqueous phase from breaking through into the collection vessel placed underneath. Organic solvent is then added to the wells.
The analyte will partition from the adsorbed aqueous phase into the organic solvent as this solvent slowly flows through the particle bed under gravity.
The organic eluate is collected in wells or tubes underneath the tips of the plate. A second addition of organic solvent is performed and the combined eluates are evaporated to dryness. The residue is reconstituted in a mobile phase compatible solution and an aliquot is injected into the chromatographic system for analysis.

Orochem Aquamatrix columns are a good alternative to Varian Toxelute, Chemelute Cartridges

Ordering Information

Aquamatrix Cartridges

Catalog #	Descriptions	Qty/Pkg.	List Price

SYDA6ML

Unbuffered Diatomaceous Earth, 6ml

100

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SYDA3ML

Unbuffered Diatomaceous Earth, 3ml

100

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